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il rhil 22 r d systems  (R&D Systems)


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    R&D Systems il rhil 22 r d systems
    Il Rhil 22 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+rhil+22+r+d+systems/Recombinant+Human+IL-10+Protein/pm37310858-202-119-121
    Average 94 stars, based on 59 article reviews
    il rhil 22 r d systems - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Modification:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Saline:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Membrane:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Western Blot:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Cell Isolation:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    SYBR Green Assay:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4 + T cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified NA/LE Mouse Anti-Human CD3 (Clone UCHT1) BD Cat# 555329; RRID: AB_395736 Purified NA/LE Mouse Anti-Human CD28 (Clone CD28.2) BD Cat# 555725; RRID: AB_396068 HIV-1 core (p24) antigen-FITC (Clone KC57) Beckman Coulter Cat# 6604665; RRID: AB_1575987 Mouse anti-human CD4 Alexa Fluor 700 (Clone RPAT4) BD Cat# 557922; RRID: AB_396943 Mouse anti-human CD4 PerCP-Cy5.5 (Clone RPAT4) BioLegend Cat# 300530; RRID: AB_893328 Mouse anti-human CD3 Pacific Blue (Clone UCHT1) BD Cat# 558117; RRID: AB_397038 Mouse anti-human CCR6 PE (Clone 11A9) BD Cat# 559562; RRID: AB_397273 Mouse anti-human CCR5 PE (Clone 2D7/ CCR5) BD Cat# 555993; RRID: AB_396279 Mouse anti human IFN-g Alexa Fluor 700 (Clone B27) BD Cat# 557995; RRID: AB_396977 Mouse anti human CXCR4 APC (Clone 12G5) Thermo Fisher Cat# 17-9999-42; RRID: AB_1724113 Mouse anti human Ki67 BV421 (Clone B56) BD Cat# 562899; RRID: AB_2686897 Rat anti human Integrinb7 PE-Cy 5 (Clone FIB504) BD Cat# 551059; RRID: AB_394026 Mouse anti human CD56 FITC (Clone HCD56) eBioscience Cat# 318304; RRID: AB_604100 Mouse anti human IL-17A PE (Clone eBio64DEC17) eBioscience Cat# 12-7179-42; RRID: AB_1724136 Mouse anti human IL-22 PE-Cyanine7 (Clone 22URTI) eBioscience Cat# 25-7229-42; RRID: AB_10853346 Mouse anti human CD8 FITC (Clone BW135/80) Miltenyi Biotech Cat# 130-080-601; RRID: AB_244336 Mouse anti human CD19 FITC (Clone LT19) Miltenyi Biotech Cat# 130-091-328; RRID: AB_244222 Mouse anti human CD45RA APCeFluor780 (Clone HI100) eBioscience Cat# 47-0458-42; RRID: AB_10853641 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (405 nm excitation) Invitrogen Cat# L34957 Rabbit anti human AhR Abcam Cat# ab190797; RRID: AB_2894707 Rabbit anti human AhR Cell Signaling Cat# 83200; RRID: AB_2800011 Rabbit anti human ARNT Cell Signaling Cat# 5537; RRID: AB_10694232 Normal Rabbit IgG Cell Signaling Cat# 2729; RRID: AB_1031062 Monoclonal anti-bActin (clone AC15) Sigma Cat# A5441; RRID: AB_476744 Bacterial and virus strains HIV-1 THRO plasmid (pTHRO.c/2626), subtype B NIH AIDS Reagent Program (Contribution of Dr. John Kappes and Dr. Christina Ochsenbauer) Cat#11745 NL4.3BaL HIV plasmid From Dr Roger J Pomerantz (Thomas Jefferson University, Philadelphia, Pennsylvania, USA) and Dr. Michel J. Tremblay (Université Laval, Québec City, QC, Canada) N/A (Continued on next page) Cell Reports 42, 112634, June 27, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Leukaphereses of ART treated and untreated people living with HIV Recruited at the Montreal Chest Institute, McGill University Health Center and Center Hospitalier de l’Université de Montréal via the collaboration of Dr Jean-Pierre Routy’s group N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s medium (DMEM) Thermo Fisher Cat# 11995065 Roswell Park Memorial Institute (RPMI) Thermo Fisher Cat# 11875-093 Penicillin/Streptomycin Gibco Cat# 15140-122 Fetal Bovine Serum (FBS) Sigma Cat# F2442 Bovine Serum Albumin (BSA) BioShop Cat# ALB001 Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 20012050 Tween 20 Thermo Fisher Cat# BP337-500 PVDF membrane Millipore Sigma Cat# ISEQ00010 RIPA Buffer 10X NEB Cat# 9806S rhIL-2 R&D Systems Cat# 202-IL-050 rhIL-10 R&D Systems Cat# 217-IL rhIL-22 R&D Systems Cat# 782-IL-010 CH-223191 Sigma Cat# C8124 FICZ Sigma Cat# SML1489 DNAzolTM Reagent Invitrogen Cat# 10503027 T7 Endonuclease I - 250 units NEB Cat# M0302S Phusion High-Fidelity DNA Polymerase NEB Cat# M0530S UltraComp ebeads eBioscience Cat# 01-2222-42 Formaldehyde 37% Sigma Cat#F79500 LC480 probe master mix Roche Cat# 4707494001 Amersham ECL Prime Western Blotting System Cytiva Cat# RPN2232 Brefeldin A Sigma Cat# B7651 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I0634 Luna Universal qPCR Master Mix NEB Cat# M3003E TMB Peroxidase Substrate Fitzgerald Cat# 42R-TB102 Streptavidin Poly-HRP40 Fitzgerald Cat# 65R-S104PHRP-200UG Critical commercial assays Memory CD4+ T cell Isolation Kit, human Miltenyi Cat#130-091-893 Fixation/Permeabilization Solution Kit (Cytofix/Cytoperm) BD Cat# 554714 Human IL-10 DuoSet ELISA R&D Systems Cat# DY217B Human IL-17A (homodimer) ELISA ReadySET-Go Thermo Fisher Cat# 88-7176-86 Human IL-22 DuoSet ELISA R&D Systems Cat# DY782 Human IFN-g ELISA Thermo Fisher Cat# 88-7316-86 QuantiTect SYBR Green RT-PCR Kit Qiagen Cat# 204245 Human T cell Nucleofector Kit Lonza Cat# VPA-1002 Rneasy Plus Mini Kit Qiagen Cat# 74136 CHIP assay kit EMD Millipore Cat# 17-295 (Continued on next page) 18 Cell Reports 42, 112634, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix NEB Cat# M3003S MyTaq Extract PCR kit Bioline Cat# BIO-21126 High-fidelity enzyme Q5 NEB Cat# M0530L QIAEX II Gel extraction kit Qiagen Cat# 20021 GeneJET PCR Purification Kit Thermo Fisher Cat# K0702 Deposited data RNA-Sequencing dataset This paper GEO: GSE198078 Original western blot images relative to Figures 1, S1 and S2.



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